| Blueprint ID | Learning Objectives |
| 6299 | Describe the role of amino acid residues at an enzymes active site mediates chemical reaction by various mechanisms |
| 6300 | Describe how the following affect enzyme reaction velocity: temperature; pH; amount of substrate; and amount of enzyme. |
| 6301 | Describe the relevance of each of the following as it applies to affecting or describing enzyme activity: substrate binding site versus active site; pH and temperature optima; zero order and first order kinetics; and turnover number (Kcat) |
| 6302 | Describe the importance of Michaelis-Menten enzyme kinetics and the significance of the constants Km and Vmax |
| 6303 | Describe the following concepts as they apply to enzyme activity and kinetics: reversible and irreversible inhibition; competitive and non-competitive inhibition; and allosteric regulation. |
| |