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Medical Education and Laboratory Support Services (MELSS)

Flow Cytometry

Data  Acquisition

Data Analysis

Experimental Design

Cell Sorting

 
The Flow Cytometry Unit at Memorial University Faculty of Medicine within Medical Education and Laboratory Support Services houses a Beckman Coulter Cytoflex Analyser and a Beckman Coulter MoFlo Astrios EQ cell sorter. 
We are located in Room H1710 in the Health Sciences Centre. The unit operates on a fee-for-service basis, and is available to both internal and external users.

Hours of Operation

Monday through Friday 9:00am -5:00pm. 
Reduced hours (9:00am - 4:30pm) June 1st through September 1st.

Equipment and Services
 
Beckman Coulter Cytoflex
  • 3 lasers - 405/488/638nm with 10 parameters (see link).
  • Data acquisition and analysis
  • Programs for analysis include Kaluza and CyteExpert
  • User training is provided for a fee.

 
Beckman Coulter MoFlo Astrios EQ
  • 3 Lasers-405/488/640 nm with 11 parameters (see link).
  • Data acquisition and analysis.
  • Cell sorting up to six-ways. Collection into microtitre plates, slides, centrifuge tubes.
  • All samples must be filtered with a 40 micron nylon mesh cell strainer prior to sorting. (BD Falcon, P/N 352235)
  • A live/dead marker is strongly recommended for cell sorting.
  • Programs for analysis include: Summit and Kaluza.

Scheduling and Data Policies

Beckman Coulter Cytoflex
Booking time is through the scheduler located in Room H1710, or call/email to book instrument time.
A requisition is required at time of use.
Users are responsible for removing data files at time of acquisition.
 
Beckman Coulter MoFlo Astrios EQ
Completed requisition forms must be submitted 24 h (minimal) prior to analysis/sort. Staff will approve and recommend run time availability. Users are responsible for removing data files at time of acquisition.
Staff operated only.
 
Biosafety Guidelines

For all biosafety guidelines refer to Memorial University Biosafety Policies and
Procedures Manual
.
All samples transported to the laboratory must be in a sealed container according to biosafety
guidelines. Laboratory coats and PPE must be worn at all times. 

Please Note: Users must add room H1710 to their current Biosafety certificate to use the facility.


Contact Persons:
 

Stephanie Tucker B.Sc., M.Sc., CEMT., LHP.
Electronic Microscopy/Flow Cytometry Supervisor
Phone: (709) 864-6556
Fax: (709) 864-4987
Email: stephant@mun.ca


   Chris Corkum B.Sc., M.Sc.
   Medical Technologist
   Phone: (709) 864-6556
   Fax: (709) 864-4987
   Email: c.corkum@mun.ca
 
Mailing Address:

Electron Microscopy/Flow Cytometry Unit, Room H1710
Medical Education and Laboratory Support Services
Faculty of Medicine
300 Prince Philip Drive
St. John’s, NL, A1B 3V6
Canada






















 
 
Copyright © 2018 Memorial University of Newfoundland.   Newfoundland and Labrador, Canada.