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Medical Education and Laboratory Support Services (MELSS)

Electron Microscopy & Flow Cytometry

Transmission electron microscopy services

Processing, embedding and cutting

Photographic printing and enlargement

Flow cytometry testing and data analysis 


About the Unit

The Electron Microscopy Unit at Memorial University Faculty of Medicine within Medical Education and Laboratory Support Services houses a Tecnai Spirit Transmission Electron Microscope.  We are a well-established biological, clinical and research electron microscopy facility.  The unit operates on a fee-for-service basis, and is available to internal and external users. 
 
Hours of Operation

Monday through Friday 8:30am - 5:00pm. 
Reduced hours (8:30-4:30) June 1st through September 1st
 
Equipment, Services, and Fees

Tecnai Spirit Transmission Electron Microscope for biological science features optical systems specifically designed to deliver high-quality imaging and resolution of biological specimens. The microscope is equipped with a 4 megapixel AMG digital camera.
 
Cost estimates can be granted upon request.
Fee Structure: Processing and embedding          $58/sample
Sectioning services (0.5 µm or 100nm)                $20/slide or grid
Microscope time                                                   $70/hr
                       
Scheduling Policies

Contact staff to schedule. 
 
Useful Links for Electron Microscopy

FEI

Jeol


MFlow Cytometry
 
About the Unit

The Flow Cytometry Unit at Memorial University Faculty of Medicine within Medical Education and Laboratory Support Services houses a Becton Dickinson FACS Calibur analyser and a Beckman Coulter MoFlo Astrios EQ cell sorter.  We are located in room H1710 in the Health Sciences Centre. The unit operates on a fee-for-service basis, and is available to both internal and external users.
 
Hours of Operation

Monday through Friday 8:30-5:00.  Reduced hours (8:30-4:30) June 1st through September 1st.
 
Equipment, Services, and Fees

Becton Dickinson FACS Calibur
2 Laser-488/640 nm with six parameters.
Data acquisition and analysis.
Programs for analysis include: Cell Quest Pro and ModFit.
User training is provided upon request.
 
Fee Structure: Trained operator data acquisition             $28/h
                        Operator assisted data acquisition           $48/h
                        Instrument training                       $80 (one-time fee)
 
 
Beckman Coulter MoFlo Astrios EQ
3 Lasers-405/488/640 nm with 11 parameters (see link).
Data acquisition and analysis.
Cell sorting up to six-ways. Collection into microtitre plates, slides, centrifuge tubes.
All samples must be filtered with a 40 micron nylon mesh cell strainer prior to sorting.
(BD Falcon, P/N 352235)
A live/dead marker is strongly recommended for cell sorting.
Programs for analysis include: Summit and Kaluza.

Fee Structure: Operator assisted  $50/h (+$10 set up fee)
 
                                                                     

Scheduling and Data Policies

Becton Dickinson FACS Calibur
Booking time is through the scheduler located in Room H1710, or call/email to book instrument time.
A requisition is required at time of use.
Users are responsible for removing data files at time of acquisition.
 
Beckman Coulter MoFlo Astrios EQ
Completed requisition forms must be submitted 24 h (minimal) prior to analysis/sort. Staff will approve and recommend run time availability. Users are responsible for removing data files at time of acquisition.
Staff operated only.
 
Biosafety Guidelines

For all biosafety guidelines refer to Memorial University Biosafety Policies and
Procedures Manual
.
All samples transported to the laboratory must be in a sealed container according to biosafety
guidelines. Laboratory coats and PPE must be worn at all times. 
 

Contact Persons:

 

 

Stephanie Tucker
Electron Microscopy/Flow Cytometry Supervisor

Phone: (709) 864-6656
Fax: (709) 777-6749
Email: stephant@mun.ca


 
Mailing Address:

Electron Microscopy/Flow Cytometry Unit, Room H1710
Medical Education and Laboratory Support Services
Faculty of Medicine
300 Prince Philip Drive
St. John’s, NL, A1B 3V6
Canada
 
   
 
Copyright © 2017 Memorial University of Newfoundland.   Newfoundland and Labrador, Canada.